# Quantum coupled mutation finder: predicting functionally or structurally important sites in proteins using quantum Jensen-Shannon divergence and CUDA programming

- Mehmet Gültas
^{1, 2}Email author, - Güncel Düzgün
^{1}, - Sebastian Herzog
^{1}, - Sven Joachim Jäger
^{1}, - Cornelia Meckbach
^{1}, - Edgar Wingender
^{2}and - Stephan Waack
^{1}Email author

**15**:96

https://doi.org/10.1186/1471-2105-15-96

© Gültas et al.; licensee BioMed Central Ltd. 2014

**Received: **10 June 2013

**Accepted: **26 March 2014

**Published: **3 April 2014

## Abstract

### Background

The identification of functionally or structurally important non-conserved residue sites in protein MSAs is an important challenge for understanding the structural basis and molecular mechanism of protein functions. Despite the rich literature on compensatory mutations as well as sequence conservation analysis for the detection of those important residues, previous methods often rely on classical information-theoretic measures. However, these measures usually do not take into account dis/similarities of amino acids which are likely to be crucial for those residues. In this study, we present a new method, the Quantum Coupled Mutation Finder (QCMF) that incorporates significant dis/similar amino acid pair signals in the prediction of functionally or structurally important sites.

### Results

The result of this study is twofold. First, using the essential sites of two human proteins, namely epidermal growth factor receptor (EGFR) and glucokinase (GCK), we tested the QCMF-method. The QCMF includes two metrics based on quantum Jensen-Shannon divergence to measure both sequence conservation and compensatory mutations. We found that the QCMF reaches an improved performance in identifying essential sites from MSAs of both proteins with a significantly higher Matthews correlation coefficient (MCC) value in comparison to previous methods. Second, using a data set of 153 proteins, we made a pairwise comparison between QCMF and three conventional methods. This comparison study strongly suggests that QCMF complements the conventional methods for the identification of correlated mutations in MSAs.

### Conclusions

QCMF utilizes the notion of entanglement, which is a major resource of quantum information, to model significant dissimilar and similar amino acid pair signals in the detection of functionally or structurally important sites. Our results suggest that on the one hand QCMF significantly outperforms the previous method, which mainly focuses on dissimilar amino acid signals, to detect essential sites in proteins. On the other hand, it is complementary to the existing methods for the identification of correlated mutations. The method of QCMF is computationally intensive. To ensure a feasible computation time of the QCMF’s algorithm, we leveraged Compute Unified Device Architecture (CUDA).

The QCMF server is freely accessible at http://qcmf.informatik.uni-goettingen.de/.

## Keywords

## Background

Multiple sequence alignments (MSAs) of homologous protein sequences give us information about two major features of the proteins of interest. The first one consists of easily detectable highly conserved residue sites that are obviously important for the structure and/or the function of the protein; while the second one corresponds to compensatory (coupled) mutations between two or more residue sites that also contain crucial information on the structural and functional basis of proteins [1]. These compensatory mutations occur according to the functional coupling of mutation positions which might be explained as one mutation in a certain site affecting a compensating mutation at another site, even if both related residue sites are distantly positioned in the protein structure [2–5]. In particular, such mutations at essential residue sites are likely to destroy protein structure which often results in loss of the protein function [6, 7]. Thus, recognition of these residue sites is as important as the strictly conserved positions for the understanding of the structural basis of protein functions and for the identification of functionally important residue positions [5, 8, 9].

Although the strictly conserved residue sites are easily detectable and interpretable in MSAs, the detection of important non-conserved compensatory mutation sites needs more complex approaches. Today, due to the simplicity and efficiency, the mutual-information-based metrics (MI-metrics) are often used to measure the co-evolutionary relationship between residue sites in MSAs [4–6, 10–13]. However, the MI-metrics strongly depend on the amino acid distributions observed in the MSA columns rather than on physical or biochemical constraints of amino acids that are likely to be crucial for the detection of functionally or structurally important compensatory mutations in a protein sequence. Further, according to the phylogenetic relationship of protein sequences and background noise, there is always a MI-value between each column pair in an MSA. Therefore, the challenging problems in bioinformatics for the detection of significant compensatory mutation signals are: i) the minimization of the influence of phylogenetic relationships of protein sequences by incorporating physical or biochemical properties of amino acids in the calculation; ii) the separation of significant signals from the background noise or unrelated pair signals.

In order to eliminate the influence of phylogeny and noise effects of MI, Dunn et al. [6] have introduced the average product correction (APC). Subtracting APC from MI, they obtained their MIp metric. However, in their model the reduction of background noise is not quantified. On the other hand, Gao et al. [13] have integrated amino acid background distribution (MIB) in the calculation of their MI-metric and focused on only 25 column pairs of each MSA with the highest normalized MI values as significant to reduce noisy effect which seems to be over-conservative, yet specific.

Large efforts have been made in the last few years to improve local-correlation-measure-based approaches to residue co-evolution when it comes to modeling effects that rely on spatial proximity (see [14] for an overview). In this case, it is necessary to disentangle direct and indirect correlations. Classical mutual information, for example, is high not only if the two sites under study are close in 3D space. Quite the contrary, any local measure of correlation, not just mutual information, is limited by the transitivity effect.

To overcome this problem, global statistical models of protein families are employed. The direct-coupling analysis (DCA) works as follows. Maximizing the entropy subject to preserving the single and pair residue frequencies observed, a joint probability distribution on all possible members of the protein family is derived. Utilizing this distribution, considerable progress in predicting residue-residue contacts in 3-dimensional protein structures was made [15–17]. Protein Sparse Inverse Covariance (PSICOV) [18] achieves disentanglement of direct and indirect correlations by inverting a residue-residue covariance matrix. In [19] further progress was made by integrating structural context and sequence co-evolution information.

There is merely a small number of methods that incorporate amino acid similarity in the prediction of functionally or structurally important sites. In this context, it is natural to partition the amino acids into chemically similar groups before applying an information-theoretic measure like the Shannon entropy [20, 21]. It was reported that many other methods fail to outperform this simple partition approach [22]. However, quantum information theory supplies a well-studied and powerful framework to integrate such similarity, where the classical Shannon entropy is swapped for the von Neumann entropy (VNE). Caffrey et al. [23] and Johansson et al. [24] have firstly introduced VNE to multiple sequence alignment analysis although they did not treat amino acid pair similarity.

Recently, a new method called Coupled Mutation Finder (CMF) has been introduced by Gültas et al. [5] to deal with phylogenetic noise as well as background signals and to quantify the error made in terms of the false discovery rate. The CMF method only focuses on BLOSUM62-dissimilar amino acid pairs as a model of compensatory mutations and integrated them in the calculation of normalized MI-metrics using a doubly stochastic matrix to transform the empirical pair distribution of the column pair. However, the CMF disregards amino acid pair similarity which can be also crucial for the detection of functionally or structurally important sites in MSAs.

In this study, we present a new method called Quantum Coupled Mutation Finder (QCMF) which extends the CMF algorithm [5] by additionally incorporating amino acid pair similarity. To this end, the QCMF invokes principles from quantum information theory, in particular for the first time in the context of MSA analysis quantum entanglement as a major resource of quantum information. Amino acid pair distributions are replaced by entangled density matrices from quantum mechanics which encompass in our case both empirical pair distributions, possibly transformed by the doubly stochastic matrix used in [5], and pair similarity. Following Capra and Singh [22] who pointed out that it is hard to improve upon metrics based on Jensen-Shannon divergences, we quantify the effect of both amino acid pair similarity and amino acid pair dissimilarity by the quantum Jensen-Shannon divergence between an entangled density matrix and the one that simply represents the amino acid pair frequencies.

The QCMF algorithm is strongly based on the matrix operations that are computationally intensive. When analyzing a single MSA, the computational time of these matrix operations rise very quickly due to the huge number of column pairs. In order to speed up the running time of the QCMF, we implemented its algorithm using Compute Unified Device Architecture (CUDA). CUDA is an efficient parallel computing architecture developed by NVIDIA that utilizes graphic processing units (GPUs) for general-purpose scientific and engineering applications [25]. Nowadays, GPUs are often used for computationally challenging problems in bioinformatics [26–29] and several other scientific fields [30–32].

## Results

Our main focus in this study was to investigate whether quantum information theory based measures could contribute beyond conventional measures to the identification of important residue sites. The Results section of this work twofold. First, to test the functionality of QCMF-significant individual residue sites we analysed the essential sites of two human proteins: epidermal growth factor receptor (EGFR) (pdb entry 2J6M) and glucokinase (GCK) (pdb entry 1V4S). The functionally and structurally important sites of both proteins have been experimentally investigated in several studies previously [33–44] and their positions were summarized in [5] as essential sites. The essential sites of these proteins consist of several non-conserved residue sites which are directly located at or near disease associated amino acid mutation (non-synonymous single nucleotide polymorphisms (nsSNPs)) sites, catalytic sites, protein binding sites and so on, each of which are likely to affect protein stability or functionality (see [5] and references therein). In addition, residue sites are defined to be in contact according to the “nearby” definition of Nussinov et al. [45] if their carbon major atoms have a distance of less than or equal to 6 Å. Consequently, we defined an individual QCMF-significant residue site as “functionally or structurally important” if it corresponds to one of these essential sites.

Second, to further investigate the performance of QCMF and to make a comparison with the previous methods (CMF [5], MIp [6], and PSICOV [18]), we selected a non-redundant set of proteins prepared by Janda et al. [46]. Although the dataset contains 216 proteins, we eliminated a few proteins due to inconsistency between corresponding MSAs and PDB files, so that we finally ended up with a dataset of 153 proteins (see Additional file 1).

The MSAs for each protein, which contain after filtering at least 125 independent sequences, were derived from the HSSP-database [47] that merges primary structure information and tertiary structure information of proteins.

Finally, we define QCMF-significant sites as follows. Let *M* be an MSA, with the protein of interest being the first row of *M*. A site pair as well as an individual site of the protein are said to be QCMF-significant with respect to the MSA *M*, if they are $({\mathbb{Q}}_{\text{ent}},M)$-significant or $({\mathbb{Q}}_{\text{sep}},M)$-significant. The latter two notions and the underlying two co-evolutionary column pair metrics ${\mathbb{Q}}_{\text{ent}}$ and ${\mathbb{Q}}_{\text{sep}}$ are defined in the Methods section. If the MSA *M* is fixed, we speak of ${\mathbb{Q}}_{\text{ent}}$-significance and ${\mathbb{Q}}_{\text{sep}}$-significance, rather than of $({\mathbb{Q}}_{\text{ent}},M)$-significance and $({\mathbb{Q}}_{\text{sep}},M)$-significance, respectively.

### QCMF

#### significant residue sites in the Human Epidermal Growth Factor Receptor (EGFR) protein

Using the MSA-specific statistical model with a false discovery rate (FDR) of 1% for both QCMF-metrics, we first determined altogether 2688 out of 26079 non-conserved column pairs as significant in corresponding MSA of human EGFR protein. 631 of these significant pairs were detected by ${\mathbb{Q}}_{\text{ent}}$-metric, and 2149 pairs were detected by ${\mathbb{Q}}_{\text{sep}}$-metric. Only 92 significant column pairs were detected by both metrics. After that, utilizing the connectivity degree technique, we predicted in total 33 residue sites in corresponding sequence of human EGFR protein as QCMF-significant (see Additional file 2). 12 of them are only ${\mathbb{Q}}_{\text{ent}}$-significant and 18 residue sites are ${\mathbb{Q}}_{\text{sep}}$-significant, the remaining 3 residue sites (A839, A882 and V902) are both ${\mathbb{Q}}_{\text{ent}}$-significant and ${\mathbb{Q}}_{\text{sep}}$-significant.

**QCMF-significant essential sites in the human EGFR protein, which are nearby either nsSNPs or strictly conserved sites**

QCMF-significant | Nearby nsSNPs, or strictly | Reference |
---|---|---|

essential sites | conserved sites | |

| 773 | [44] |

| 773 | [44] |

| 829 | [44] |

| 829 | [44] |

| 835 | |

| 892 | [44] |

| 861 | |

| 796 | - |

| 884 | - |

| 898 | - |

| 880 | - |

| 906 | - |

| 906 | - |

According to the essential sites of human EGFR protein, published in [5], we have shown altogether the structural or functional importance of 25 QCMF-significant sites. The remaining 8 significant residue sites (G729, T851, G779, Q820, M825, L927, G930, Y944) do not fall into essential sites and the reason for their significance and their importance in the EGFR protein is currently unclear.

#### QCMF-significant residue sites in the Human Glucokinase (GCK) protein

Like human EGFR protein, applying the MSA-specific statistical model with a FDR of 1% for both QCMF-metrics we identified a total of 9853 out of 69645 non-conserved column pairs as significant in the human GCK protein (pdb entry 1V4S). 6070 of them were $({\mathbb{Q}}_{\text{ent}},M)$-significant and 4232 were detected as $({\mathbb{Q}}_{\text{sep}},M)$-significant. Only 449 column pairs were detected as significant with respect to both metrics. Thereupon using the connectivity degree technique, we determined altogether 64 residue sites in the human GCK protein as QCMF-significant (see Additional file 3). 30 of them are determined as ${\mathbb{Q}}_{\text{ent}}$-significant and further 30 significant residue sites are determined as ${\mathbb{Q}}_{\text{sep}}$-significant. Only four residue sites (T82, G223, V253, and G407) are significant based on both metrics.

*V*62,

*M*210,

*Y*215 are allosteric sites by themselves [41] and the T209M, G223S and S453del are related to disease associated nsSNP positions. In addition, there are further five QCMF-significant sites (F123L, G162D, G175R, T228M, and E300K,Q) that have been verified as nsSNP positions through annotation databases and previous experimental studies [38–40, 42, 43, 51]. The structural localization of these 18 QCMF-significant sites (contact sites and nsSNPs positions) are illustrated inFigure 3.

**QCMF-significant essential sites in the human GCK protein, which are nearby either nsSNPs or strictly conserved sites**

QCMF-significant | Nearby nsSNPs or strictly | Reference |
---|---|---|

essential sites | conserved sites | |

| 36 | |

| 147 | |

| 78 | - |

| 81 | - |

| 81 | |

| 85 | [38] |

| 130 | [40] |

| 147 | |

| 150 | |

| 154 | [39] |

| 154 | [39] |

| 119 | [43] |

| 164 | |

| 162 | |

| 182 | |

| 147 | |

| 147 | [43] |

| 223 | |

| 223 | |

| 234 | |

| 257 | |

| 274 | |

| 274 | [43] |

| 278 | [43] |

| 291 | [43] |

| 295 | [43] |

| 295 | |

| 377 | |

| 377 | [43] |

| 377 | [43] |

| 382 | [43] |

| 382 | [43] |

| 385 | [43] |

| 385 | |

| 226 | |

| 416 | [40] |

| 444 | [39] |

| 444 | [39] |

In total, we have demonstrated here that according to the essential sites of GCK, 62 out of 64 QCMF- significant sites are functionally or structurally important for human GCK protein. The remaining two significant residue sites V89 and N283 do not overlap with essential sites and the reason for their significance and their role in the GCK protein is still unclear.

### Individual residue site comparison between QCMF-significant sites and previous CMF-significant sites

We compared QCMF-significant residue sites for both human EGFR and GCK proteins with the significant residue sites given in [5] of the previous CMF-method. The CMF-method detected for both human proteins, 43 sites in EGFR and 72 sites in GCK as significant.

For the EGFR protein we found that the QCMF-significant residue sites Q791, Q820, G824, K860, Y891, T892, Y900, T909 overlap with results of the CMF-method. Interestingly, one of the unconfirmed residue sites, the Q820, has been predicted by both QCMF-method and CMF-method as significant.

For GCK protein, we observed that in total 24 QCMF- significant sites (T60, T82, N83, F123, F148, T149, F152, H156, F171, N180, T206, T209, T228, E236, G260, L271, S281, N283, Q287, G294, E300, T332, F419 and E443) were also determined by the CMF-method as significant. Although both methods detected residue site N283 as significant, it corresponds to one of the unconfirmed residue sites for GCK, currently.

The CMF has been developed using normalized mutual information (MI) measures in order to detect important residue positions in MSAs. The method mainly focuses on significant BLOSUM62-dissimilar amino acid signals as a model of compensatory mutations and integrates them in the calculation of normalized MI-metrics. As a consequence of mainly taking into account dissimilar amino acid signals, an important part of CMF-significant sites were verified as disease associated nsSNP positions and just a small part of them were located at or near the catalytic sites, allosteric sites and binding sites in both proteins.

Moreover, when statistically evaluating both methods, we have observed that the QCMF significantly outperforms the QCMF-method. The QCMF reaches an improved performance in identifying essential sites from MSAs of both proteins with a significantly higher Matthews correlation coefficient (MCC) value of 0.215 whereas the CMF reaches only a MCC value of 0.133.

### Significant residue pair comparison

To analyze whether the quantum-information-theory-based measures proposed in this study complements the coventional methods for the detection of correlated (co-evolutionary) mutations, we made pairwise comparisons between our new QCMF, MIp [6], PSICOV [18], and CMF [5].

All four methods take as input an MSA satisfying certain admissibility criteria. The problem is that QCMF and CMF output the set of QCMF-significant sites and CMF-significant sites of *M*’s reference protein, respectively, whereas PSICOV and MIp result in sets of important residue pairs. To make these outputs comparable, we extend them in all cases.

Let ${\mathcal{V}}_{\text{QCMF}}$ denote the output of QCMF on any admissible MSA *M*. We extend this set to what we call the QCMF-significant residue network ${\mathcal{N}}_{\text{QCMF}}:=\left({\mathcal{V}}_{\text{QCMF}},{\mathcal{E}}_{\text{QCMF}}\right)$ of *M* as follows. Any two elements of ${\mathcal{V}}_{\text{QCMF}}$ are connected by an undirected edge belonging to ${\mathcal{E}}_{\text{QCMF}}$ if and only if the corresponding column pair is QCMF-significant.

The CMF-significant residue network ${\mathcal{N}}_{\mathit{\text{CMF}}}$ is analogously defined.

In order to get a sufficiently large number MIp-significant and PSICOV-significant residue pairs, for every input MSA we simply took the top-ranking 10% as MIp-significant and PSICOV-significant, respectively.

We then utilized the connectivity degree technique in the same way as we did for CMF and QCMF to calculate the set of MIp-significant sites ${\mathcal{V}}_{\text{MIp}}$ and the set of PSICOV-significant sites ${\mathcal{V}}_{\text{PSICOV}}$.

For all four methods we used the 90th, the 95th and the 99th percentile as *cut-off* values.

Finally, the edge sets ${\mathcal{E}}_{\text{MIp}}$ and ${\mathcal{E}}_{\text{PSICOV}}$ were determined by full analogy with the calculation of ${\mathcal{E}}_{\text{QCMF}}$ and ${\mathcal{E}}_{\text{CMF}}$. Thus we obtained the MIp-significant residue network ${\mathcal{N}}_{\text{MIp}}$ and the PSICOV-significant residue network ${\mathcal{N}}_{\text{PSICOV}}$.

*i*=1,2,…,153. Summing up the 153 numbers in each of these groups results in the numbers $\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{QCMF}}^{\left(i\right)}\right|$, $\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{CMF}}^{\left(i\right)}\right|$, $\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{PSICOV}}^{\left(i\right)}\right|$, $\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{MIp}}^{\left(i\right)}\right|$, $\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{QCMF}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{MIp}}^{\left(i\right)}\right|$, $\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{QCMF}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{PSICOV}}^{\left(i\right)}\right|$, $\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{QCMF}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{CMF}}^{\left(i\right)}\right|$, $\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{MIp}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{PSICOV}}^{\left(i\right)}\right|$, $\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{MIp}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{CMF}}^{\left(i\right)}\right|$ and $\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{PSICOV}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{CMF}}^{\left(i\right)}\right|$, which are displayed in Tables 3 and 4.

**Total number of edges in method-dependent significant residue networks with respect to various connectivity degree cut-offs**

Total number of edges in significant residue networks | |||
---|---|---|---|

Connectivy degree cut-off | 90 %th percentile | 95 %th percentile | 99 %th percentile |

$\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{QCMF}}^{\left(i\right)}\right|$ | 82561 | 20411 | 435 |

$\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{MIp}}^{\left(i\right)}\right|$ | 90636 | 24094 | 1454 |

$\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{PSICOV}}^{\left(i\right)}\right|$ | 80489 | 21596 | 1088 |

$\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{CMF}}^{\left(i\right)}\right|$ | 87208 | 23893 | 936 |

**Total number of edges in two networks of different type with respect to various connectivity degree cut-offs**

Total number of common edges in two networks of different type | |||
---|---|---|---|

Connectivy degree cut-off | 90 %th percentile | 95 %th percentile | 99 %th percentile |

$\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{QCMF}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{MIp}}^{\left(i\right)}\right|$ | 898 | 77 | 0 |

$\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{QCMF}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{PSICOV}}^{\left(i\right)}\right|$ | 735 | 64 | 0 |

$\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{QCMF}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{CMF}}^{\left(i\right)}\right|$ | 4036 | 474 | 1 |

$\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{MIp}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{PSICOV}}^{\left(i\right)}\right|$ | 9094 | 1488 | 11 |

$\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{MIp}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{CMF}}^{\left(i\right)}\right|$ | 3343 | 474 | 6 |

$\sum _{i=1}^{153}\left|{\mathcal{E}}_{\text{PSICOV}}^{\left(i\right)}\cap {\mathcal{E}}_{\text{CMF}}^{\left(i\right)}\right|$ | 2618 | 368 | 2 |

Table 3 shows that all methods detect with the same connectivity degree cut-off a comparable number of edges in the corresponding significant residue network.

Table 4 highly suggests that all four methods carry distinct information. The overlap between any two of them is less than or equal to 10%. This indicates that, under the assumption that each of them models important aspects of co-evolution, they complement each other perfectly. In particular, this is true for QCMF as a quantum-information-science-based service compared with the other three established tools that are based on conventional methods.

### Implementation of QCMF: Parallel computing using CUDA

The computation of both QCMF metrics (Equations 7 and 8) is strongly based on matrix operations. Therefore, we implement QCMF algorithm using CUDA [25] which is very suitable to perform large number of vector and matrix operations in real time. This results in a dramatic reduction of computational time of QCMF.

Applying the QCMF algorithm for human EGFR protein with CPU alone and with CUDA acceleration, the average computational time of a column pair was 0.7117 seconds and 0.0301 seconds, respectively. Similarly, for human GCK protein, the average computational time of a column pair was 0.6977 seconds with CPU alone and 0.0299 seconds with CUDA acceleration. Consequently, the algorithm took ∼310 minutes for human EGFR protein and ∼811 minutes for GCK protein with CPU alone. On the other hand, applying the CUDA acceleration it took only ∼13 minutes for EGFR and ∼39 minutes for GCK protein. The comparison between the average times indicates that the required computational time of QCMFalgorithm with the CUDA acceleration was significantly faster than with CPU alone (approximately more than 23 times faster).

## Methods

We predict important sites of a protein by detecting co-evolving residues. Our measures of co-evolution are quantum-Jensen-Shannon-divergence-based metrics of column pairs of a multiple sequence alignment, with the protein under study being the reference row. The quantum Jensen-Shannon divergence in turn has the von Neumann entropy as main building block.

The von Neumann entropy was originally defined in the framework of quantum mechanics. We elucidate it in the subsequent section as far as it is necessary to understand our methods. Researchers interested in learning more are referred to the excellent textbook due to Vedral [57]. A comprehensive reference book was published by Nielsen and Chuang [58].

This section is organized as follows. In the first four subsections we recapitulate techniques developed in [5] which we leverage in this study. This concerns the definition of significant site pairs and of significant individual sites, the preparation of the training data set used, and the computation of a doubly stochastic matrix *D* as our model of compensatory mutations on grounds of two counting matrices *C*_{alt} and *C*_{null}. These two matrices also form the basis of the two amino acid pair similarity matrices ${\mathcal{A}}_{\text{ent}}$ and ${\mathcal{A}}_{\text{sep}}$, which in turn give rise to our new quantum-information-science-based metrics ${\mathbb{Q}}_{\text{ent}}$ and ${\mathbb{Q}}_{\text{sep}}$. The last four subsection are dedicated to their definitions.

### Significant column pairs and significant position with respect to a certain metric

Let *M* be an MSA, where the protein of interest is represented by *M*’s first row, and let
be a metric which assigns to every MSA column pair (*γ*_{1},*γ*_{2}) a real number $\mathbb{E}\left({\gamma}_{1},{\gamma}_{2}\right)\in [0,1]$. We call
a *co-evolutionary column pair metric* if it models a biologically meaningful co-evolutionary signal: The larger the metric value on (*γ*_{1},*γ*_{2}), the more likely co-evolution between position *γ*_{1} and position *γ*_{2} has occurred.

Let ${\hat{p}}_{(i,j)}$ be the empirical relative amino acid pair frequency of the *i*-th and the *j*-th amino acid in column pair (*γ*_{1},*γ*_{2}), where *i*,*j*=1,2,…,20. (When choosing a row of this column pair by pure chance, acid pair (*i*,*j*) is drawn with probability ${\hat{p}}_{(i,j)}$.) In the subsequent subsection we recapitulate the way developed in [5] to identify significant columns and significant column pairs with respect to
.

where $\mathbb{H}({\gamma}_{1},{\gamma}_{2})$, $\mathbb{H}\left({\gamma}_{1}\right)$, and $\mathbb{H}\left({\gamma}_{2}\right)$ denote the Shannon entropy of the empirical pair distribution ${\left({\hat{p}}_{(i,j)}\right)}_{i,j=1,2,\dots ,20}$ of the column pair (*γ*_{1},*γ*_{2}) and its two marginals.

In order to identify significant column pairs of the MSA under study with respect to the metric
, in [5] we have pointed out, that the distribution of
can be regarded as a mixture of a background *β*-distribution *F*_{0}, an unrelated pair distribution *G*_{1}, and a distribution *G*_{2} of presumably co-evolving pairs.

The *p*-values $1-{F}_{0}\left(\mathbb{E}\right)$ are then uniformly distributed over [ 0,1] given the underlying
-values are *F*_{0}-distributed. In contrast, *p*-values tend to zero or one, if
-values are *G*_{2}-distributed or *G*_{1}-distributed, respectively.

If, moreover, there is a sub-interval of [ 0,1] which contains only data from the background distribution, on grounds of a result due to Storey and Tibshirani [59, 60] we determined in [5] an MSA-dependent threshold for -values. A column pair is said to be $(\mathbb{E},M)$-significant, if its -value is above the threshold, where the false discovery rate is bounded by a predefined constant.

We applied that model in this study.

We utilized the connectivity degree technique, introduced in [12] and developed further in [5], in order to define the $(\mathbb{E},M)$-significance of individual residue sites. The connectivity degree of a position *γ*_{1} is the number of positions *γ*_{2} so that the site pair (*γ*_{1},*γ*_{2}) is $(\mathbb{E},M)$-significant. A site of the protein of interest is then called $(\mathbb{E},M)$-significant, if its connectivity degree *cut-off* exceeds the 90-th percentile.

### Training data set and pre-processing

Following [5], a redundancy free set of more than 35000 protein structures is our starting point. This collection was compiled in Rainer Merkl’s Lab at the University of Regensburg. The protein structures were taken from the protein data base (http://www.pdb.org/). The PISCES services [61] was applied to assess proteins on sequence similarity and equality of 3D-data. The related MSAs were gathered from the HSSP data base (http://swift.cmbi.ru.nl/gv/hssp/).

Taking pattern from [12], we filtered every MSA obtained as follows. First, highly similar and dissimilar sequences were deleted to ensure that the sequence identity between any two sequences is at least 20% and no more than 90%. Second, we removed strictly conserved residue columns, where the percentage of identical residues is greater than 95%. Third, we eliminated the residue columns which contain more than 25% gaps. Finally, we discarded all MSAs with less than 125 sequences. More than 17000 MSAs survived the last filtering step. We used approximately 1700 MSAs published in [5] as our *training data set* which we randomly chose from this set.

### Setting up the counting matrices *C*_{alt} and *C*_{null}

The entries of the two matrices are frequencies of pair substitutions calculated from our training data set described in the foregoing subsection. Informally spoken, matrix *C*_{alt} models the signal, whereas *C*_{null} reflects the background.

In line with [5], we calculated a signal and a null set of column pairs. The signal set consists of all $(\mathcal{U},M)$-significant column pairs, where *M* ranges over all training MSA. The null set consists of sufficiently many column pairs randomly chosen from every training MSA. For both the signal set and the null set we computed a symmetric 400×400 integer-valued matrix of frequencies of pair substitutions *C*_{alt} and *C*_{null}. To this end, the method used to compute BLOSUM62 matrices [62] is applied to count residue pair substitutions in MSA column pairs rather than residue substitution in columns.

### Computing a doubly stochastic matrix *D*

According to [5], a pair ((*a*_{
i
},*a*_{
j
}),(*a*_{
k
},*a*_{
l
})) of amino acid pairs is said to be a *formal dissimilar compensatory mutation*, if the BLOSUM62 score both of (*a*_{
i
},*a*_{
k
}) and (*a*_{
j
},*a*_{
l
}) is negative.

*C*

_{alt}and

*C*

_{null}, we define the matrix

*C*

_{CompMut}by

*φ*

_{CompMut}((

*a*

_{ i },

*a*

_{ j }),(

*a*

_{ k },

*a*

_{ l }))=1 if and only if either (

*a*

_{ i },

*a*

_{ j })=(

*a*

_{ k },

*a*

_{ l }) or ((

*a*

_{ i },

*a*

_{ j }),(

*a*

_{ k },

*a*

_{ l })) is a formal dissimilar compensatory mutation and

By normalizing *C*_{CompMut}, we obtain a symmetric matrix *P*_{CompMut}. For *a*_{
i
},*a*_{
j
},*a*_{
k
},*a*_{
l
} ranging over all amino acids, *P*_{CompMut}((*a*_{
i
},*a*_{
j
}),(*a*_{
k
},*a*_{
l
})) represents an empirical probability distribution on pairs of amino acid pairs.

where ${P}_{\text{CompMut}}^{\mathrm{b}}\left({a}_{i},{a}_{j}\right)$ is the marginal distribution of *P*_{CompMut}.

Having set all negative entries of *S*_{CompMut} to zero, the doubly stochastic matrix *D* is computed by means of the canonical iterated row-column normalization procedure [63].

The doubly stochastic *D* is used to linearly transform empirical amino acid pair distributions of column pairs. If the pair distribution is regarded as a 400-dimensional row vector, matrix *D* is multiplied from the right. If then, for example, the resulting distribution is plugged into Equation 1, column pairs containing formal dissimilar compensatory mutations the *D*-transition probability of which is relatively large tend to be up-scaled.

The idea of the subsequent subsections is to design a model of MSA column pairs that takes formal dissimilar compensatory mutations regarded as pair dissimilarities as well as pair similarities into account. The challenge is to implement this in a way such that these two effects interfere but do not interact. This is necessary since a similarity relation is transitive, whereas a dissimilarity relation is not.

### Setting up the two counting matrices *C*_{ent} and *C*_{sep}

*C*

_{ent}and

*C*

_{sep}from

*C*

_{alt}and

*C*

_{null}which form the basis of our new metrics ${\mathbb{Q}}_{\text{ent}}$ and ${\mathbb{Q}}_{\text{sep}}$. The intuition behind

*C*

_{ent}is that the component-wise BLOSUM62-based pair similarity is rescaled, whereas

*C*

_{sep}leads to a new amino acid pair similarity.

*φ*

_{ent}((

*a*

_{ i },

*a*

_{ j }),(

*a*

_{ k },

*a*

_{ l }))=1 if and only if either (

*a*

_{ i },

*a*

_{ j })=(

*a*

_{ k },

*a*

_{ l }) or the following two conditions are satisfied. First, the amino acids

*a*

_{ i }and

*a*

_{ k }as well as the amino acids

*a*

_{ j }and

*a*

_{ l }are BLOSUM62-similar. Second,

where *φ*_{sep}((*a*_{
i
},*a*_{
j
}),(*a*_{
k
},*a*_{
l
}))=1 if and only if either (*a*_{
i
},*a*_{
j
})=(*a*_{
k
},*a*_{
l
}) or Equation 2 is satisfied.

### Calculating the two amino acid pair similarity matrices

Recall that a matrix *A* is positive definite (positive semi-definite), if there is an orthogonal matrix *U* (defining property *U*^{−1}=*U*^{
T
}) such that *U* *A* *U*^{
T
} is a diagonal matrix, where the coefficients in the main diagonal are strictly positive (non-negative).

Let us call a 400×400-matrix
a *amino acid pair similarity matrix*, if
is positive definite and the entries in the main diagonal are equal to 1, whereas the off-diagonal elements ${\mathcal{A}}_{(g,h),(i,j)}$ ((*g*,*h*)≠(*i*,*j*)) are greater than or equal to 0, but less than 1.

The entries of an amino acid pair similarity matrix
are interpreted as follows. The closer ${\mathcal{A}}_{(g,h),(i,j)}$ to 1, the more similar are the amino acid pairs (*g*,*h*) and (*i*,*j*).

*C*be either

*C*

_{ent}or

*C*

_{sep}. We define

where ((*g*,*h*),(*i*,*j*)) ranges over all possible 160000 indices of pairs of amino acid pairs including the main diagonal, and *α*∈(0,1) was set to 0.1 in order to enhance the effect of similarity.

*B*is not in any case positive definite, we finally set

which is justified by the transitivity of similarity. That way the amino acid similarity matrices ${\mathcal{A}}_{\text{ent}}$ and ${\mathcal{A}}_{\text{sep}}$ are obtained from the counting matrices *C*_{ent} and *C*_{sep}, respectively.

Amino acid pair similarity matrices generalize amino acid similarity matrices used by Johansson et al. [24] for evaluating amino acid conservation.

### Modeling MSA column pairs and single columns by means of density matrices

Let (*γ*_{1},*γ*_{2}) be a column pair of a multiple sequence alignment, let ${\left({\hat{p}}_{(i,j)}\right)}_{i,j=1,2,\dots ,20}$ be the empirical amino acid pair distribution in these columns, let ${\left({\hat{q}}_{(i,j)}\right)}_{i,j=1,2,\dots ,20}$ be the linear transform of ${\left({\hat{p}}_{(i,j)}\right)}_{i,j=1,2,\dots ,20}$ by the doubly stochastic matrix *D*, and let
be an amino acid pair similarity matrix.

Recall, that the trace of a matrix is the sum of its coefficients in the main diagonal.

Taking pattern from quantum mechanics, we model column pair (*γ*_{1},*γ*_{2}) by a positive semi-definite 400×400-matrix the trace of which is equal to 1, a so-called *density matrix*. Regarding the two distributions ${\left({\hat{p}}_{(i,j)}\right)}_{i,j\phantom{\rule{0.3em}{0ex}}=\phantom{\rule{0.3em}{0ex}}1,2,\dots ,20}$ and ${\left({\hat{q}}_{(i,j)}\right)}_{i,j\phantom{\rule{0.3em}{0ex}}=\phantom{\rule{0.3em}{0ex}}1,2,\dots ,20}$ as 400×400-diagonal matrices the main diagonal of which are formed by the probabilities ${\hat{p}}_{(i,j)}$ and ${\hat{q}}_{(i,j)}$, respectively, we integrate the classical model into the quantum-mechanics-based one.

where ${\hat{r}}_{(i,j)}$ is either ${\hat{p}}_{(i,j)}$ or ${\hat{q}}_{(i,j)}$ (*i*,*j*=1,2,…,20). Using this denotation, the diagonal density matrices considered in the preceding paragraph are equal to some $\rho \left(\hat{r},\mathbb{1}\right)$, where$\mathbb{1}$ is the 400×400-identity matrix.

In this study, we regard individual MSA columns only as components of column pairs. In the classical case, where MSA-column pair (*γ*_{1},*γ*_{2}) is modeled by an MSA-dependent amino acid pair distribution $\hat{r}$ (either ${\left({\hat{p}}_{(i,j)}\right)}_{i,j\phantom{\rule{0.3em}{0ex}}=\phantom{\rule{0.3em}{0ex}}1,2,\dots ,20}$ or some derivative), the columns *γ*_{1} and *γ*_{2} are represented by the corresponding marginals ${\hat{r}}_{1}$ and ${\hat{r}}_{2}$ of $\hat{r}$.

_{2}(

*ρ*) and tr

_{1}(

*ρ*) of

*ρ*. They are 20×20 density matrices defined by

where *i*,*j*=1,2…,20. As opposed to the indices of the marginals, matrix tr_{1}(*ρ*) models column *γ*_{2}, whereas matrix tr_{2}(*ρ*) represents column *γ*_{1}.

### Defining our two new metrics ${\mathbb{Q}}_{\text{ent}}$ and ${\mathbb{Q}}_{\text{sep}}$

To begin with, we define the von Neumann entropy VNE(*ρ*) of a diagonal density matrix *ρ* as the Shannon entropy of its main diagonal coefficients regarded as a probability distribution.

*ρ*is that there exists an orthogonal matrix

*U*such that

*U*

*ρ*

*U*

^{ T }is a diagonal density matrix, where the diagonal elements are uniquely determined up to their order. Thus we are justified to finally define

where *U* is an orthogonal matrix diagonalizing *ρ* in a way just mentioned.

In principle, the following holds true. The larger the off-diagonal coefficients of the similarity matrix
, the smaller the von Neumann entropy of the density matrix according to Equation 4 compared with the Shannon entropy of the probability distribution ${\hat{r}}_{(i,j)}$ (*i*,*j*=1,2,…,20).

*ρ*and

*σ*of the same dimension, we make use of the quantum Jensen-Shannon divergence:

It can be shown that 0≤QJSD(*ρ*∥*σ*)≤1, where 0 is attained if and only if the two density matrices *ρ* and *σ* are equal. As oppose to the case of Equation 1, we have thus avoided a normalization.

We are now in a position to define our new two metrics for a certain column pair of a given MSA. As before, the amino acid pair distribution $\hat{q}$ is given by $\hat{p}\xb7D$, where *D* is the 400×400 doubly stochastic matrix described above, $\hat{p}$ is the empirical pair distribution of these two columns, and$\mathbb{1}$ is the 400×400-identity matrix.

(see Equation 4). This metric measures the difference between a density matrix combining rescaled amino acid pair similarity with dissimilar compensatory mutations and the empirical amino acid pair distribution. The index “*ent*” indicates that here we make use of quantum entanglement, which in turn is a major resource of quantum information science. (Entangled 400×400-density matrices are those that cannot be represented as a convex combination of Kronecker products of 20×20-density matrices. Note, that the Kronecker product of density matrices is the analog of the classical product of probability distributions).

The density operator $\rho \left(\hat{p},{\mathcal{A}}_{\text{sep}}\right)$ is entangled. However, before finally calculating the metric, we separate the columns of the pair by applying the two partial trace operators.

Using the example of the human EGFR protein (PDB-Entry 2J6M), Figure 6 illustrates that the method we developed in [5] to determine significant column pairs is well-applicable for both ${\mathbb{Q}}_{\text{ent}}$ and ${\mathbb{Q}}_{\text{sep}}$. The results presented in this work prove that ${\mathbb{Q}}_{\text{ent}}$ as well as ${\mathbb{Q}}_{\text{sep}}$ are powerful co-evolutionary column pair metrics.

## Discussion

Grosse *et al.* observed in [64] that the Jensen-Shannon divergence (JSD) can be interpreted as mutual information between two (or more) random sources in a special setting particularly appropriate to discriminate between these sources. This is what we need when it comes to predicting important protein sites in an MSA-based approach. It might explain the findings of Capra and Singh [22] on the predictive power of JSD. These two articles encouraged us to utilize quantum Jensen-Shannon divergence (QJSD) in this study. As a side effect, a normalization is not necessary, since quantum Jensen-Shannon divergence, like its classical counterpart, ranges over the real interval [ 0,1].

Several studies have confirmed the fact that detecting coupled MSA-columns is extremely useful in the prediction of important protein sites (see e.g. [4–6, 10–13, 65–70]). When using information-theoretic metrics, there is no doubt that it is reasonable to incorporate amino acid pair dissimilarity as well as amino acid similarity in a consistent way such that similarity decreases entropy, whereas dissimilarity increases it. This kind of consistency is important, since entropy is the fundamental building block for most of those metrics. In particular, the Jensen-Shannon divergence between two probability mass functions (pmfs) *p* and *q* equals $\mathbb{H}(1/2(p+q\left)\right)-1/2\left(\mathbb{H}\right(p)+\mathbb{H}(q\left)\right)$.

In [5] an amino acid pair dissimilarity model for compensatory mutations is presented. A doubly stochastic matrix transforms the empirical amino acid pair distribution of a column pair.

Rescaled pair similarity of BLOSUM62-similar pairs is to capture an aspect of coupled MSA column pairs orthogonal to the phenomenon of dissimilar compensatory mutations. It models the amino acid pair transition preferences within those column pairs on the average. As suggested by Caffrey *et al.*[23] as well as Johansson *et al.*[24], it is promising to incorporate them within the framework of quantum information theory. Therein, density matrices replace pmfs. The counterpart of the entropy of a pmf is the von Neumann entropy (VNE) of a density matrix (see Equation 5). QJSD corresponds then exactly to JSD (see Equation 6).

The challenge was to complement the model presented in [5] by additionally incorporating amino acid pair similarity in a way that the two effects interfere but do not interact. We model an MSA column pair by means of a 400×400-density matrix, rather than amino acid pair distributions. This provides us with the opportunity to utilize the notion of entanglement, which in turn is a major resource of quantum information. In our model, partial traces play the role of the marginals in the classical case. Pair similarity is reflected by means of positive definite pair similarity matrices (see Equation 3), where positive definiteness, which is a key property of density matrices, can only be ensured by using transitivity of similarity. Since there is no transitivity of dissimilarity, we kept dissimilarity apart from that similarity matrix. Instead, we carried over the CMF dissimilarity model of [5]. Similarity matrix and transformed amino acid pair distribution are joined together by means of Equation 4 in the final step of our density matrix design. That way we minimize the interaction between the two effects of dissimilarity and similarity.

In order to eliminate the noise and to define an MSA-dependent threshold for significant column pairs, we followed the line of [5]. The model presented there seems to be of universal applicability. The same is true for the connectivity degree model introduced in [12] and further developed in [5]. Combining them results in a reliable and robust method to determine significant residues.

The results we present in this study show that the vast majority of QCMF-significant residue sites are closely related to functionality and structural stability of both human EGFR and GCK proteins. 10 significant residue sites in EGFR and 19 significant sites in GCK are established as functionally important since they are directly located at or close to catalytic sites, allosteric sites and binding sites which are crucial for maintaining protein functions and for understanding the underlying molecular mechanism (see Figures 1,2,3,4). Additionally, 2 significant sites in EGFR and 8 significant sites in GCK (three of them are also in contact with allosteric sites in GCK) are related to disease associated nsSNP regions of both proteins. As has been noted in [5], most disease-causing mutations at these positions in corresponding sequences destroy structural features of proteins, thus affecting protein stability and often results in loss of protein function.

Although the importance of almost all QCMF-significant sites are verified through essential sites of both human proteins, there are still eight and two unconfirmed significant sites in EGFR and GCK proteins, respectively, which do not fall into essential sites. It is interesting to note that some of these unconfirmed sites are also referred as significant by CMF [5]. We therefore believe that most of these unconfirmed sites identified by our present method may have an importance for the function and structural stability of both proteins notwithstanding the absence of previous experimental data. A further comparison reveals that the overlaps between the results of the QCMF method and the CMF method are quite low, indicating that both methods detect considerably different sets of residue sites as functionally and structurally important. The comparison results clearly show that considering similar and dissimilar amino acid signals simultaneously, our present method is more sensible to catalytic, allosteric and binding sites, while only focusing on dissimilar signals the previous method deals successfully with nsSNP positions in proteins.

The final comparison between QCMF and CMF on EGFR and GCK proteins is made by inspecting several connectivity degree cut-offs. We initially set it to the 90-th percentile at which CMF reaches its maximal MCC value. Going through all possible *n*-th percentiles for *n*=80,81,…,99, QCMF reaches its maximal MCC value of 0.231 if *n*=88. What we got can be summarized as follows. On the one hand QCMF shows a better performance than CMF in identifying important residue sites. On the other hand QCMF complements CMF. This is because of the fact that the method of QCMF is more information rich than that of CMF. QCMF simultaneously uses similar and dissimilar amino acid pair signals, whereas CMF’s method focuses only on amino acid pair dissimilarity.

To confirm the educated guess that QCMF complements conventional methods both from information theory and statistics, we applied QCMF, CMF [5], MIp [6] and PSICOV [18] to the 153 MSAs described at the beginning of the Results section. In sum, each of these methods detects different residue pairs as important, where the pairwise overlap is bounded from above by 10%. The reason for that is that the four methods model different aspects of amino acid pair co-evolution. Consequently, they carry distinct information.

To further improve the specificity of QCMF it is promising to combine its quantum-information-theory-based framework with the direct pair distribution derived in DCA (see e.g. [15] or [16]).

## Conclusions

In this work, we report a new method, QCMF, applying principles of quantum information theory. In contrast to the previous method CMF which focused on dissimilar amino acid signals, QCMF simultaneously models similar and dissimilar amino acid pair signals in the detection of functionally or structurally important sites. QCMF includes two metrics based on quantum Jensen-Shannon divergence. While the first metric measures compensatory mutations between pairs of columns, the second metric considers the sequence conservation of columns. Results show that QCMF reaches an improved performance in identifying important sites from MSAs and it predicts a quite different set of residue sites as functionally and structurally important (in comparison to the previous method). Further, results indicate that the residue sites found by QCMF are more sensible to catalytic sites, allosteric sites and binding sites than those found by the previous method. On the top of that, a pairwise comparison with existing methods shows that QCMF is complementary to them when it comes to predicting co-evolving residue site pairs.

## Declarations

### Acknowledgements

We thank Rainer Merkl from Regensburg for his helpful advice and insights at early stages of this project and for comments on the methods.

Mehmet Gültas acknowledges financial support by the Deutsche Forschungsgemeinschaft (DFG: WA 766/7-1).

## Authors’ Affiliations

## References

- Gloor GB, Martin LC, Wahl LM, Dunn SD: Mutual information in protein multiple sequence alignments reveals two classes of Coevolving positions. Biochemistry. 2005, 44 (19): 7156-7165. 10.1021/bi050293e. [http://pubs.acs.org/doi/abs/10.1021/bi050293e]. [PMID: 15882054],View ArticlePubMedGoogle Scholar
- Wilson K, Walker J: Principles and Techniques of Biochemistry and Molecular Biology. 2010, New York: Cambridge University PressView ArticleGoogle Scholar
- Altschuh D, Lesk AM, Bloomer AC, Klug A: Correlation of co-ordinated amino acid substitutions with function in viruses related to tobacco mosaic virus. J Mol Biol. 1987, 193 (4): 693-707. 10.1016/0022-2836(87)90352-4.View ArticlePubMedGoogle Scholar
- Martin LC, Gloor GB, Dunn SD, Wahl LM: Using information theory to search for co-evolving residues in proteins. Bioinformatics. 2005, 21 (22): 4116-4124. 10.1093/bioinformatics/bti671.View ArticlePubMedGoogle Scholar
- Gültas M, Haubrock M, Tüysüz N, Waack S: Coupled mutation finder: a new entropy-based method quantifying phylogenetic noise for the detection of compensatory mutations. BMC Bioinformatics. 2012, 13: 225-10.1186/1471-2105-13-225. [http://www.biomedcentral.com/1471-2105/13/225],View ArticlePubMed CentralPubMedGoogle Scholar
- Dunn SD, Wahl LM, Gloor GB: Mutual information without the influence of phylogeny or entropy dramatically improves residue contact prediction. Bioinformatics. 2008, 24 (3): 333-340. 10.1093/bioinformatics/btm604.View ArticlePubMedGoogle Scholar
- Chakrabarti S, Panchenko AR: Structural and functional roles of Coevolved sites in proteins. PLoS ONE. 2010, 5: e8591-10.1371/journal.pone.0008591. [doi:10.1371%2Fjournal.pone.0008591].,View ArticlePubMed CentralPubMedGoogle Scholar
- Sandler I, Abu-Qarn M, Aharoni A: Protein co-evolution: how do we combine bioinformatics and experimental approaches?. Mol BioSyst. 2013, 9: 175-181. 10.1039/c2mb25317h. [doi:10.1039/C2MB25317H],View ArticlePubMedGoogle Scholar
- DePristo MA, Weinreich DM, Hartl DL: Missense meanderings in sequence space: a biophysical view of protein evolution. Nat Rev Genet Nat Publishing Group. 2005, 6 (9): 678-687. [doi:10.1038/nrg1672],View ArticleGoogle Scholar
- Atchley WR, Wollenberg KR, Fitch WM, Terhalle W, Dress AW: Correlations among amino acid sites in bHLH protein domains: an information theoretic analysis. Mol Biol Evol. 2000, 17: 164-10.1093/oxfordjournals.molbev.a026229.View ArticlePubMedGoogle Scholar
- Tillier ER, Lui TW: Using multiple interdependency to separate functional from phylogenetic correlations in protein alignments. Bioinformatics. 2003, 19 (6): 750-755. 10.1093/bioinformatics/btg072. [http://bioinformatics.oxfordjournals.org/content/19/6/750.abstract],View ArticlePubMedGoogle Scholar
- Merkl R, Zwick M: H2r: Identification of evolutionary important residues by means of an entropy based analysis of multiple sequence alignments. BMC Bioinformatics. 2008, 9: 151-10.1186/1471-2105-9-151. [http://www.biomedcentral.com/1471-2105/9/151],View ArticlePubMed CentralPubMedGoogle Scholar
- Gao H, Dou Y, Yang J, Wang J: New methods to measure residues coevolution in proteins. BMC Bioinformatics. 2011, 12: 206-10.1186/1471-2105-12-206. [http://www.biomedcentral.com/1471-2105/12/206].,View ArticlePubMed CentralPubMedGoogle Scholar
- de Juan D, Pazos F, Valencia A: Emerging methods in protein co-evolution. Nat Rev Genet. 2013, 14: 249-261.View ArticlePubMedGoogle Scholar
- Morcos F, Pagnani A, Lunt B, Bertolino A, Marks DS, Sander C, Zecchina R, Onuchic JN, Hwa T, Weigt M: Direct-coupling analysis of residue coevolution captures native contacts across many protein families. Proc Nat Acad Sci. 2011, 108 (49): E1293-E1301. 10.1073/pnas.1111471108. [http://www.pnas.org/content/108/49/E1293.abstract],View ArticlePubMed CentralPubMedGoogle Scholar
- Marks DS, Colwell LJ, Sheridan R, Hopf TA, Pagnani A, Zecchina R, Sander C: Protein 3D structure computed from evolutionary sequence variation. PLoS ONE. 2011, 6 (12): e28766-10.1371/journal.pone.0028766.View ArticlePubMed CentralPubMedGoogle Scholar
- Cheng RR, Morcos F, Levine H, Onuchic JN: Toward rationally redesigning bacterial two-component signaling systems using coevolutionary information. Proc Nat Acad Sci. 2014, [http://www.pnas.org/content/early/2014/01/17/1323734111.abstract],Google Scholar
- Jones DT, Buchan DWA, Cozzetto D, Pontil M: PSICOV: precise structural contact prediction using sparse inverse covariance estimation on large multiple sequence alignments. Bioinformatics. 2012, 28 (2): 184-190. 10.1093/bioinformatics/btr638.View ArticlePubMedGoogle Scholar
- Kamisetty H, Ovchinnikov S, Baker D: Assessing the utility of coevolution-based residue-residue contact predictions in a sequence- and structure-rich era. Proc Nat Acad Sci. 2013, 110 (39): 15674-15679. 10.1073/pnas.1314045110. [http://www.pnas.org/content/110/39/15674.abstract],View ArticlePubMed CentralPubMedGoogle Scholar
- Williamson R: Information theory analysis of the relationship between primary sequence structure and ligand recognition among a class of facilitated transporters. J Theor Biol. 1995, 174: 179-188. 10.1006/jtbi.1995.0090.View ArticlePubMedGoogle Scholar
- Mirny J, Shakhnovich E: Universally conserved position in protein folds: reading evolutionary signals about stability, folding, kinetics, and function. J Mol Biol. 1999, 291: 10930-10935.View ArticleGoogle Scholar
- Capra JA, Singh M: Predicting functionally important residues from sequence conservation. Bioinformatics. 2007, 23 (15): 1875-1882. 10.1093/bioinformatics/btm270.View ArticlePubMedGoogle Scholar
- Caffrey DR, Somaroo S, Hughes JD, Mintseris J: Huang ES: Are protein-protein interfaces more conserved in sequence than the rest of the protein surface?. Protein Sci. 2004, 13: 190-2002. 10.1110/ps.03323604.View ArticlePubMed CentralPubMedGoogle Scholar
- Johansson F, Toh H: Relative von Neumann entropy for evaluating amino acid conservation. J Bioinformatics Comput Biol. 2010, 08 (05): 809-823. [http://www.worldscientific.com/doi/abs/10.1142/S021972001000494X],View ArticleGoogle Scholar
- NVIDIA CUDA Zone. [http://www.nvidia.com/object/cuda_home_new.html],
- Liu Y, Wirawan A, Schmidt B: CUDASW++ 3.0: accelerating Smith-Waterman protein database search by coupling CPU and GPU SIMD instructions. BMC Bioinformatics. 2013, 14: 117-10.1186/1471-2105-14-117. [http://www.biomedcentral.com/1471-2105/14/117],View ArticlePubMed CentralPubMedGoogle Scholar
- Manavski S, Valle G: CUDA compatible GPU cards as efficient hardware accelerators for Smith-Waterman sequence alignment. BMC Bioinformatics. 2008, 9 (Suppl 2): S10-10.1186/1471-2105-9-S2-S10.View ArticlePubMed CentralPubMedGoogle Scholar
- Lui Y, Maskell D, Schmidt B: CUDASW++: optimizing Smith-Waterman sequence database searches for CUDA-enabled graphics processing units. BMC Res Notes. 2009, 2: 73-10.1186/1756-0500-2-73.View ArticleGoogle Scholar
- Wirawan A, Kwoh C, Hieu N, Schmidt B: CBESW: Sequence alignment on the Playstation 3. BMC Bioinformatics. 2008, 9: 377-10.1186/1471-2105-9-377.View ArticlePubMed CentralPubMedGoogle Scholar
- Ufimtsev I, Martinez T: Graphical processing units for quantum chemistry. Comput Sci Eng. 2008, 10 (6): 26-34.View ArticleGoogle Scholar
- Stone J, Hardy D, Ufimtsev I, Schulten K: GPU-accelerated molecular modeling coming of age. J Mol Graph Model. 2010, 29 (2): 116-125. 10.1016/j.jmgm.2010.06.010.View ArticlePubMed CentralPubMedGoogle Scholar
- Michalakes J, Vachharajani M: GPU acceleration of numerical weather prediction. Parallel Process Lett. 2008, 18 (4): 531-548. 10.1142/S0129626408003557.View ArticleGoogle Scholar
- Dixit A, Yi L, Gowthaman R, Torkamani A, Schork NJ, Verkhivker GM: Sequence and structure signatures of cancer mutation Hotspots in protein Kinases. PLoS ONE. 2009, 4 (10): e7485-10.1371/journal.pone.0007485. [doi:10.1371%2Fjournal.pone.0007485],View ArticlePubMed CentralPubMedGoogle Scholar
- Yun CH, Boggon TJ, Li Y, Woo MS, Greulich H, Meyerson M, Eck MJ: Structures of lung cancer-derived EGFR mutants and inhibitor complexes: mechanism of activation and insights into differential inhibitor sensitivity. Cancer Cell. 2007, 11 (3): 217-227. 10.1016/j.ccr.2006.12.017. [http://www.sciencedirect.com/science/article/pii/S1535610807000281],View ArticlePubMed CentralPubMedGoogle Scholar
- Zhang H, Berezov A, Wang Q, Zhang G, Drebin J, Murali R, Greene MI: ErbB receptors: from oncogenes to targeted cancer therapies. J Clin Invest. 2007, 117 (8): 2051-2058. 10.1172/JCI32278. [http://www.jci.org/articles/view/32278],View ArticlePubMed CentralPubMedGoogle Scholar
- Lynch TJ, Bell DW, Sordella R, Gurubhagavatula S, Okimoto RA, Brannigan BW, Harris PL, Haserlat SM, Supko JG, Haluska FG, Louis DN, Christiani DC, Settleman J, Haber DA: Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to Gefitinib. N Engl J Med. 2004, 350 (21): 2129-2139. 10.1056/NEJMoa040938. [http://www.nejm.org/doi/full/10.1056/NEJMoa040938],View ArticlePubMedGoogle Scholar
- Balius TE, Rizzo RC: Quantitative prediction of fold resistance for inhibitors of EGFR. Biochemistry. 2009, 48 (35): 8435-8448. 10.1021/bi900729a. [http://pubs.acs.org/doi/abs/10.1021/bi900729a]. [PMID: 19627157],View ArticlePubMed CentralPubMedGoogle Scholar
- Tinto N, Zagari A, Capuano M, De Simone A, Capobianco V, Daniele G, Giugliano M, Spadaro R, Franzese A, Sacchetti L: Glucokinase gene mutations: structural and genotype-phenotype analyses in MODY children from South Italy. PLoS ONE. 2008, 3 (4): e1870-10.1371/journal.pone.0001870. [http://dx.plos.org/10.1371\%2Fjournal.pone.0001870],View ArticlePubMed CentralPubMedGoogle Scholar
- Capuano M, Garcia-Herrero CM, Tinto N, Carluccio C, Capobianco V, Coto I, Cola A, Iafusco D, Franzese A, Zagari A, Navas MA, Sacchetti L: Glucokinase (GCK) mutations and their characterization in MODY2 children of Southern Italy. PLoS ONE. 2012, 7 (6): e38906-10.1371/journal.pone.0038906. [doi:10.1371%2Fjournal.pone.0007485],View ArticlePubMed CentralPubMedGoogle Scholar
- Garcia-Herrero CM, Rubio-Cabezas O, Azriel S, Gutierrez-Nogues A, Aragones A, Vincent O, Campos-Barros A, Argente J, Navas MA: Functional characterization of MODY2 mutations highlights the importance of the fine-tuning of glucokinase and its role in glucose sensing. PLoS ONE. 2012, 7: e30518-10.1371/journal.pone.0030518. [doi:10.1371%2Fjournal.pone.0038906],View ArticlePubMed CentralPubMedGoogle Scholar
- Kamata K, Mitsuya M, Nishimura T, ichi Eiki J, Nagata Y: Structural basis for allosteric regulation of the monomeric allosteric enzyme human glucokinase. Structure. 2004, 12 (3): 429-438. 10.1016/j.str.2004.02.005. [http://www.sciencedirect.com/science/article/pii/S0969212604000474],View ArticlePubMedGoogle Scholar
- Hamosh A, Scott AF, Amberger JS, Bocchini CA, McKusick VA: Online Mendelian Inheritance in Man (OMIM), a knowledgebase of human genes and genetic disorders. Nucleic Acids Res. 2005, 33 (suppl 1): D514-D517. [http://nar.oxfordjournals.org/content/33/suppl_1/D514.abstract],PubMed CentralPubMedGoogle Scholar
- Reichert J, Sühnel J: The IMB Jena image library of biological macromolecules: 2002 update. Nucleic Acids Res. 2002, 30: 253-254. 10.1093/nar/30.1.253. [http://nar.oxfordjournals.org/content/30/1/253.abstract],View ArticlePubMed CentralPubMedGoogle Scholar
- Flicek P, Amode MR, Barrell D, Beal K, Brent S, Chen Y, Clapham P, Coates G, Fairley S, Fitzgerald S, Gordon L, Hendrix M, Hourlier T, Johnson N, Kähäri A, Keefe D, Keenan S, Kinsella R, Kokocinski F, Kulesha E, Larsson P, Longden I, McLaren W, Overduin B, Pritchard B, Riat HS, Rios D, Ritchie GRS, Ruffier M, Schuster M, et al: Ensembl 2011. Nucleic Acids Res. 2011, 39 (suppl 1): D800-D806. [http://nar.oxfordjournals.org/content/39/suppl_1/D800.abstract],View ArticlePubMed CentralPubMedGoogle Scholar
- Keskin O, Tsai CJ, Wolfson H, Nussinov R: A new, structurally nonredundant, diverse data set of protein-protein interfaces and its implications. Protein Sci. 2004, 13 (4): 1043-1055. 10.1110/ps.03484604. [doi:10.1110/ps.03484604],View ArticlePubMed CentralPubMedGoogle Scholar
- Janda JO, Busch M, Kuck F, Porfenenko M, Merkl R: CLIPS-1D: analysis of multiple sequence alignments to deduce for residue-positions a role in catalysis, ligand-binding, or protein structure. BMC Bioinformatics. 2012, 13: 55-10.1186/1471-2105-13-55. [http://www.biomedcentral.com/1471-2105/13/55],View ArticlePubMed CentralPubMedGoogle Scholar
- Sander C, Schneider R: Database of homology derived protein structures and the structural meaning of sequence alignment. Proteins. 1991, 9 (1): 56-69. 10.1002/prot.340090107.View ArticlePubMedGoogle Scholar
- Porter CT, Bartlett GJ, Thornton JM: The Catalytic site Atlas: a resource of catalytic sites and residues identified in enzymes using structural data. Nucleic Acids Res. 2004, 32 (suppl 1): D129-D133. [http://nar.oxfordjournals.org/content/32/suppl_1/D129.abstract],View ArticlePubMed CentralPubMedGoogle Scholar
- Sherry ST, Ward MH, Kholodov M, Baker J, Phan L, Smigielski EM, Sirotkin K: dbSNP: the NCBI database of genetic variation. Nucleic Acids Res. 2001, 29: 308-311. 10.1093/nar/29.1.308. [http://nar.oxfordjournals.org/content/29/1/308.abstract],View ArticlePubMed CentralPubMedGoogle Scholar
- Dixit A, Yi L, Gowthaman R, Torkamani A, Schork NJ, Verkhivker GM: Sequence and structure signatures of cancer mutation hotspots in protein kinases. PLoS ONE. 2009, 4 (10): e7485-10.1371/journal.pone.0007485. [doi:10.1371%2Fjournal.pone.0030518].,View ArticlePubMed CentralPubMedGoogle Scholar
- Valentinova L, Beer NL, Stanik J, Tribble ND, van de Bunt M, Huckova M, Barrett A, Klimes I, Gasperikova D, Gloyn AL: Identification and functional Characterisation of novel glucokinase mutations causing maturity-onset diabetes of the young in Slovakia. PLoS ONE. 2012, 7 (4): e34541-10.1371/journal.pone.0034541. [doi:10.1371%2Fjournal.pone.0007485],View ArticlePubMed CentralPubMedGoogle Scholar
- Bosma W, Cannon J, Playoust C: The Magma algebra system. I. The user language. J Symbolic Comput. 1997, 24 (3–4): 235-265. [doi:10.1006/jsco.1996.0125]. [Computational algebra and number theory (London, 1993)],View ArticleGoogle Scholar
- Anderson E, Bai Z, Bischof C, Blackford S, Demmel J, Dongarra J, Du Croz J, Greenbaum A, Hammarling S, McKenney A, Sorensen D: LAPACK Users’ Guide. 1999, Philadelphia, PA: Society for Industrial and Applied MathematicsView ArticleGoogle Scholar
- An updated set of basic linear algebra subprograms (BLAS). ACM Trans Math Softw. 2002, 28 (2): 135-151. 10.1145/567806.567807. [http://doi.acm.org/10.1145/567806.567807],
- Goto K, Geijn RAvd: Anatomy of high-performance matrix multiplication. ACM Trans Math Softw. 2008, 34 (3): 12:1-12:25. [http://doi.acm.org/10.1145/1356052.1356053],View ArticleGoogle Scholar
- Lifflander J, Evans GC, Arya A, Kale L: Dynamic Scheduling for Work Agglomeration on Heterogeneous Clusters. Parallel and Distributed Processing Symposium Workshops PhD Forum (IPDPSW), 2012 IEEE 26th International. 2012, 2404-2413. doi:10.1109/IPDPSW.2012.297,View ArticleGoogle Scholar
- Vedral V: Introduction to Quantum Information Science (Oxford Graduate Texts). 2006, New York: Oxford University Press Inc.View ArticleGoogle Scholar
- Nielsen MA, Chuang IL: Quantum Computation and Quantum Information. 2000, Cambridge University PressGoogle Scholar
- Storey JD, Tibshirani R: Statistical significance for genomewide studies. Proc Acad Sci. 2003, 100: 9440-9445. 10.1073/pnas.1530509100.View ArticleGoogle Scholar
- Walsh B: Multiple comparisons: Bonferroni corrections and false discovery rates. Lecture Notes EEB 581, Department of Ecology and Evolutionary Biology, University of Arizona, 2004Google Scholar
- Wang G, Jr RLD: PISCES: recent improvements to a PDB sequence culling server. Nucleic Acids Res. 2005, 33 (Web-Server-Issue): 94-98.View ArticleGoogle Scholar
- Henikoff S, Henikoff JG: Amino acid substitution matrices from protein blocks. Proc Nat Acad Sci. 1992, 89 (22): 10915-10919. 10.1073/pnas.89.22.10915. [http://www.pnas.org/content/89/22/10915.abstract],View ArticlePubMed CentralPubMedGoogle Scholar
- Cappellini V, Sommer HJ, Bruzda W, Zyczkowski K: Random bistochastic matrices. J Phys A: Math Theor. 2009, 42: 23-View ArticleGoogle Scholar
- Grosse I, Bernaola-Galván P, Carpena P, Román-Roldán R, Oliver J, Stanley HE: Analysis of symbolic sequences using the Jensen-Shannon divergence. Phys Rev E. 2002, 65: 041905-[http://link.aps.org/doi/10.1103/PhysRevE.65.041905],View ArticleGoogle Scholar
- Göbel U, Sander C, Schneider R, Valencia A: Correlated mutations and residue contacts in proteins. Proteins-Struct Funct Genet. 1994, 18 (4): 309-317. 10.1002/prot.340180402.View ArticlePubMedGoogle Scholar
- Neher E: How frequent are correlated changes in families of protein sequences?. Proc Nat Acad Sci. 1994, 91: 98-102. 10.1073/pnas.91.1.98. [http://www.pnas.org/content/91/1/98.abstract],View ArticlePubMed CentralPubMedGoogle Scholar
- Pollock DD, Taylor WR: Effectiveness of correlation analysis in identifying protein residues undergoing correlated evolution. Protein Eng. 1997, 10 (6): 647-657. 10.1093/protein/10.6.647. [http://peds.oxfordjournals.org/content/10/6/647.abstract],View ArticlePubMedGoogle Scholar
- Lockless SW, Ranganathan R: Evolutionarily conserved pathways of energetic connectivity in protein families. Science. 1999, 286 (5438): 295-299. 10.1126/science.286.5438.295. [http://www.sciencemag.org/content/286/5438/295.abstract],View ArticlePubMedGoogle Scholar
- Dekker JP, Fodor A, Aldrich RW, Yellen G: A perturbation-based method for calculating explicit likelihood of evolutionary co-variance in multiple sequence alignments. Bioinformatics. 2004, 20 (10): 1565-1572. 10.1093/bioinformatics/bth128.View ArticlePubMedGoogle Scholar
- Codoner FM, Fares M: Why should we care about molecular coevolution?. Evol Bioinform. 2008, 4: 29-38.Google Scholar

## Copyright

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.