XLPM: efficient algorithm for the analysis of protein-protein contacts using chemical cross-linking mass spectrometry
© Jaiswal et al.; licensee BioMed Central Ltd. 2014
Published: 21 October 2014
Chemical cross-linking is used for protein-protein contacts mapping and for structural analysis. One of the difficulties in cross-linking studies is the analysis of mass-spectrometry data and the assignment of the site of cross-link incorporation. The difficulties are due to higher charges of fragment ions, and to the overall low-abundance of cross-link species in the background of linear peptides. Cross-linkers non-specific at one end, such as photo-inducible diazirines, may complicate the analysis further. In this report, we design and validate a novel cross-linked peptide mapping algorithm (XLPM) and compare it to StavroX, which is currently one of the best algorithms in this class.
We have designed a novel cross-link search algorithm -XLPM - and implemented it both as an online tool and as a downloadable archive of scripts. We designed a filter based on an observation that observation of a b-ion implies observation of a complimentary y-ion with high probability (b-y filter). We validated the b-y filter on the set of linear peptides from NIST library, and demonstrate that it is an effective way to find high-quality mass spectra. Next, we generated cross-linked data from an ssDNA binding protein, Rim1with a specific cross-linker disuccinimidyl suberate, and a semi-specific cross-linker NHS-Diazirine, followed by analysis of the cross-linked products by nanoLC-LTQ-Orbitrap mass spectrometry. The cross-linked data were searched by XLPM and StavroX and the performance of the two algorithms was compared. The cross-links were mapped to the X-ray structure of Rim1 tetramer. Analysis of the mixture of NHS-Diazirine cross-linked 15N and 14N-labeled Rim1 tetramers yielded 15N-labeled to 14N-labeled cross-linked peptide pairs, corresponding to C-terminus-to-N-terminus cross-linking, demonstrating interaction between different two Rim1 tetramers. Both XLPM and StavroX were successful in identification of this interaction, with XLPM leading to a better annotation of higher-charged fragments. We also put forward a new method of estimating specificity and sensitivity of identification of a cross-linked residue in the case of a non-specific cross-linker.
The novel cross-link mapping algorithm, XLPM, considerably improves the speed and accuracy of the analysis compared to other methods. The quality selection filter based on b-to-y ions ratio proved to be an effective way to select high quality cross-linked spectra.
Mapping of protein-protein interactions (PPIs) is an important, yet challenging analytical problem. This is also a potential clinical need, as having a detailed PPI information may aid in finding novel targets for therapeutic intervention . Thus far, mainstream methods of PPI mapping include affinity-purification-mass-spectrometry (AP-MS), yeast-two-hybrid (Y2H), or mammalian-two hybrid methods. PPI information obtained from these experiments is deposited into online databases, such as BioGRID . However, it takes a lot of effort to map the full interactome using these approaches. Also, as researches are starting to ask questions about interactome dynamics , we need faster and more effecting ways to map PPI networks. We suggest that PPI sampling using chemical cross-linking is an attractive alternative to AP-MS and Y2H methods.
In our recent review article on the subject, we presented a case for the use of short-length cross-linkers of broad specificity for the purpose of sampling PPIs and discussed existing challenges and limitations . We argued that a short, broadly specific cross-linker could yield a more comprehensive interactome analysis and, in some cases, the mapping of individual PPI interfaces. The main difficulties include identification of cross-linked peptides in the background of non-cross-linked species and also, discriminating between inter- and intra-protein cross-links and assessing the false positive rate of cross-linked site identification. The other technical problem is the computational expense of allowing for non-specific cross-linking (i.e. cross-linked to any amino acid). We proposed the following steps to overcome these difficulties. Step 1 - starting at the scale of few proteins, develop an efficient filtering and scoring system to analyze cross-linking data, allowing for non-specific cross-linking at one end. Step 2 - expand to the large scale removing all specificity requirements. In this publication we complete the Step 1 and build a foundation to move onto Step 2.
For a scale of few proteins, it is easy to design a cross-link search algorithm, when one is relying on a database consisting of pair-wise combinations of peptides from interacting proteins. Next, from this database the list of possible cross-linked precursors is prepared. There are many software tools that use this approach: CLPM , xComb , GPMAW , X!Link , StavroX , MassMatrix . However, all of them perform rather poorly when non-specificity is allowed. Also, not all of those algorithms take a full advantage of MS/MS spectra for defining the position of the cross-linked amino acid residues.
In this paper, we present a new algorithm, XLPM, which uses a novel method to allow effective use of the tandem MS information for the cross-link analysis without dramatically affecting the speed when non-specificity is allowed. We used StavroX as a benchmark to evaluate the XLPM performance, because, in our hands, StavroX produced the most robust and reliable data amongst all the programs we evaluated. We used the photo labile diazirine cross-linker SDA  on a Rim1 tetramer to evaluate the XLPM. Rim1 is a single strand DNA binding protein localizing to yeast mitochondria. Our data suggest a previously unreported tetramer-to-tetramer interaction. The functional analysis of this interaction and its biological implications will be presented elsewhere. Here, we focused mainly on the computational analysis. Additionally we propose a novel method to validate the cross-linked site assignment: by relaxing specificity of a known, selective cross-linker. For this purpose we used the Rim1 cross-linked dataset obtained using DSS.
The algorithm and the program workflow of XLPM are shown in Additional file 1. XLPM receives information of protein sequences, a spectra file in mgf format, digestion enzyme, and cross-linker, static and variable modifications of amino acids, missed cleavage level, precursor ion tolerance in ppm and fragment ion tolerance in Daltons. XLPM generate temporary database of cross-linked pairs from given protein sequences. First, amino acids that may be cross-linked are marked in the protein sequence. A list of digested fragment sequences is generated. A missed cleavage is forced. In other words, digested fragments having only one amino acid at the C terminal, which can be cross-linked, are removed from the list. The digested fragments devoid of any amino acids that can be cross-linked are also filtered out. A database of cross-linked pairs is generated from the list of digested fragments including the change of the mass due to any amino acid modification.
A file in a mascot generic format (mgf) is uploaded and read; data are extracted from it and parsed into precursor ions and fragment ions. The precursor ion masses are compared with the masses of cross-linked pairs in the database generated previously with the user defined error tolerance. Each matched cross-linked product is further analyzed using its MS/MS fragmentation pattern and scored.
b-y filter and analysis of fragment ion spectra
Workflow and implementation
The database contains pre-compiled data of digestive enzymes, cross-linkers, modifications, and amino acids. A new enzyme, cross-linker or custom modification can easily be added to the database and used in subsequent analyses. The algorithm is compatible with digestive enzym cleaving at either end of one or more amino acids. The algorithm can also handle all kinds of amino acid modifications for which precise changes in the masses are known. XLPM is better than many existing tools, as it can accommodate specific, semi-specific and non-specific cross-linkers. The algorithm can also perform isotopic analysesusing15N-labled proteins.
The XLPM score is a representation of the proportion of matched b-ions and y-ions, using the following formula:
Nmb and Nmy are numbers of theoretical b ions and y ions matched to the CID spectrum, respectively. Ntb and Nty are numbers of total possible b ions and y ions, respectively. Ryb is the median ratio of complementary y ions to b ions for the charge of the precursor ion as calculated from NIST the data, described in the previous segment.
The following materials were purchased from Thermo Fisher Scientific or its subsidiaries: HPLC-grade acetonitrile, formic acid, HEPES, Tris, NaCl, EDTA, MgCl2, SDS, KOH, β-mercaptoethanol, acrylamide, bisacrylamide, formamide, xylene cyanol, bromphenol blue, urea, glycerol, SDA cross-linker, formaldehyde, DSS, Gel-Code blue stain, and Zeba-Spin Desalting columns for buffer exchange.
Recombinant helicase domain of Pif1, full-length Pif1, and Rim1-C-terminal 6xHis proteins used in this work were purified as described in  K29A Rim1-C-terminal 6xHis mutant was purified from E. Coli, using the same isolation protocols as for wild-type Rim1, established in . Throughout the article, we call Rim1-C-terminal 6xHis as wild type Rim1, and K29A Rim1-C-terminal -6xHis as K29A Rim1. The proteins without 6xHis tag as labeled as "no-tag", when appropriate.
To metabolically label Rim1, E. Coli expressing Rim1 was grown on media prepared with 15N-ammonium chloride as the sole source of nitrogen . Next, the 15N-labeled Rim1 was purified according to the previously established protocol .
Cross-linking reactions using DSS
To the 2X stock of Rim1 protein (6 µM of Rim1 tetramer), was added equal volume of 5 mM DSS in a cross-linking reaction buffer. Final concentrations of the reagents in the reaction mix were: 25 mM HEPES, 100 mMNaCl, 10% Glycerol, 3 µM of Rim1 tetramer, 2.5 mM DSS, pH = 7.5. The reaction was incubated at room temperature for 40 min, with mild agitation. Next, the quenching buffer (Tris, pH 8) was added to the final concentration of 50 mM. Using Zeba Spin Desalting columns (Thermo Fisher), the buffer was exchanged into 25 mM HEPES, 100 mMNaCl, pH 7.5. 90% protein recovery was verified by Bradford assay. The mixture was boiled in SDS loading buffer, and the products were resolved on 5-15% gradient SDS-PAGE.
Cross-linking reactions using SDA
Prior to the cross-linking experiments, the proteins were thawed on ice, and using Zeba Spin Desalting columns, the storage buffer was exchanged to the cross-linking reaction buffer (25 mM HEPES, 100 mM NaCl, 10% Glycerol, pH 7.5). In all of the cross-linking reactions, the final concentrations of Rim1 (tetramer) were 3 µM. To the 2X stock of Rim1 protein in the cross-linking buffer (6 µM of Rim1), SDA stock solution in DMSO was added (50 µM SDA and 0.5% DMSO final concentrations). The NHS reaction was carried out at 25 °C for 30 min followed by the addition of 1M Tris, pH 8.0 (50 mM final Tris) and the additional incubation at 25 °C for 5 min. Next, to remove the unreacted cross-linker, the buffer was exchanged with fresh cross-linking buffer using the Zeba Spin Desalting columns. A 90-95% percent protein recovery was verified by Bradford assay. For the second step of the SDA-cross-linking reaction, an equal volume of the 2X Rim1 protein or 2X Pif1 protein, to be reacted, was added to the NHS-reacted mix. UV-cross-linking was performed in a Strata linker 1800 using coated bulbs (365 nm emission maximum) at a 2 cm distance from the bulbs in black, flat-bottomed 96-well plates. 4X SDS buffer was added to the reactions and the reaction products were resolved on a5-15% gradient SDS-PAGE. The cross-linked band was excised, in-gel digested and analyzed by the NanoLC-LTQ-Orbitrap-Velos mass spectrometry, as described below.
NanoLC-LTQ-Orbitrap mass spectrometry, Identification of cross-linked peptides and Mass spectrometry analysis
Gel slices were destained in 50% methanol, 100 mM ammonium bicarbonate, followed by reduction in 10 mM Tris[2-carboxyethyl] phosphine and alkylation in 50 mM iodoacetamide. Gel slices were then dehydrated in acetonitrile, followed by addition of 100 ng porcine trypsin in 100 mM ammonium bicarbonate and incubation at 37°C for 12-16 hours. Peptide products were then acidified in 0.1% formic acid. Tryptic peptides were separated by reverse phase Jupiter Proteo resin (Phenomenex) on a 100 × 0.075 mm column using a nano Acquity UPLC system (Waters). Peptides were eluted using a 30 min gradient from 98:2 to 40:60 buffers A:B ratio. [Buffer A = 0.1% formic acid, 0.05% acetonitrile; buffer B = 0.1% formic acid, 75% acetonitrile.] Eluted peptides were ionized by electrospray (1.9 kV) followed by MS/MS analysis using collision induced dissociation on an LTQ Orbitrap Velos mass spectrometer (Thermo).
To detect the cross-linked peptides, an LTQ-Orbitrap-Velos was used with the following parameters: 1) the three most intense precursors were subjected to MS/MS analysis - each of the precursors was analyzed consecutively by HCD (7,500 resolution), CID with the analysis of the fragments in OrbiTrap (FT-CID 7,500 resolution), and regular CID; 2) each sample was run twice, the first time allowing all of the charges, and the second time allowing only precursors of charge 4 and higher to be fragmented; 3) the precursor selection intensity threshold was set to 5000; 4) for the 15N-labeled samples, only the HCD fragmentation of the 10 most intense precursor ions at 7,500 resolution was used.
Data analysis and peak lists preparation
Peaks lists in a mascot generic format were generated from the raw files using Proteo Wizard tools. The same sets of parameters were used for both XLPM and StavroX. An input file with tags and values was made for XLPM. To evaluate a possible impact of charge deconvolution of fragment spectra we used spectral refinement function available in the PEAKS v7 software suite (Bioinformatics Solutions Inc.). The spectral deconvolution did increase the scores obtained through StavroX by annotating more highly-charge fragments, while no improvement was observed in the case of XLPM, because XLPM already had most of the highly charged fragments (data not shown).
Protein structure visualization
To construct, manipulate, and visualize the X-ray structure we used PyMOL (The PyMOL Molecular Graphics System, Version 22.214.171.124 Schrödinger, and LLC).
XLPM web interface
Validation of the b-y filter on a set of linear peptides
The algorithm, comparing MS/MS spectra of precursor ion with all b ions and complementary y ions, uses b-y ion filter and is based on the observation, "Detection of a particular b ion with the charge less than that of the precursor implies a high probability of detection of the complementary y-ion with the remaining charge." As the amino acid sequences of proteins and the details of cross-linker are known, the algorithm can analyse the results without matching the entire MS/MS spectra. Matching all b ions and complementary y ions reduces the work of the algorithm considerably, making it fast and efficient.
We analysed the NIST (National Institute of Standard and Technology) (http://peptide.nist.gov/) library of annotated ion trap spectra to validate the b-y filter. For an MS/MS spectrum of each precursor, b-ions having charges less than that of the corresponding precursor ion were identified, and the spectrum was then scanned for the complementary y ions with the remaining charge. The ratio of the number of such y ions to the number of b ions was calculated for the spectrum of each precursor ion. All the ratios were plotted separately for each of the charges; two, three, four, five and higher.
Mapping of the Rim1 tetramer DSS cross-links
StavroX results for DSS cross-linking of Rim1 tetramer
StavroX results for DSS cross-linking of Rim1 dimer
XLPM results for DSS cross-linking of Rim1 tetramer
XLPM results for DSS cross-linking of Rim1 dimer
Rim1 tetramer cross-linking using the non-specific cross-linker SDA
SDA cross-linker connects lysine at one end to any amino acid at the other. We developed XLPM to include non-specific analysis with no limitations. Rim1 tetramer was cross-linked using the SDA (Figure 5, right panel ) and the cross-linked products were analysed using XLPM. XLPM successfully identified nine cross-linked candidates in each of the two bands. This result illustrates the ability of XLPM not only to identify the peptides cross-linked using a non-specific cross-linker, but also XLPM's ability to distinguish between the sites of cross-linking. Two confident sites were identified by XLPM from the SDA-cross-linked spectra: N-terminus MDFSK to YLKSIAPQR peptide (Additional file 5), and N-terminus MDFSK to C-terminal peptide LEADAEGQENAASSEHHHHHH.
Identification of interaction between Rim1 tetramers using 15N-labeling
Novel features of XLPM compared to other algorithms for cross-link analysis
The b-y filter implemented in the current version of XLPM, makes the cross-link identification considerably faster compared to existing algorithms. This is because only a sub-set of fragment ions is calculated in theoretical spectra and considered for identification. Even for linear peptides, using the analysis of the set of NIST MS/MS spectra, we showed that there is a high correlation between the presence of a b-ion and the presence of the complementary y-ion in the same spectrum. This is in line with the observations by Frank et. al. that b and y ions are highly correlated in doubly charged peptides . Furthermore, Tabb et. al. noted that y-ions are usually higher in intensity than the complementary b ions, in the case of tryptic peptides. Therefore, if a b-ion is observed in an MS/MS spectrum, it is highly likely that the complimentary y-ion will also be observed. While it is certainly true that having a complimentary set of b and y ions is not a requirement for peptide identification, what is likely - is that when such condition occurs it indicates that the MS/MS spectrum is of very high quality, leading to a high-scoring identification. The b-y filter, therefore, could be thought as a quality filter. Our analysis of NIST data suggests that even a regular data-base search of a proteomics dataset may benefit from such a filter. We also expect that for the non-linear, cross-linked peptides, the b-y filter should perform just as well as for linear peptides. Indeed, a fragmentation spectrum of a regular linear peptide precursor in a typical experiment using collisional excitation, conforms to a "mobile proton model": when peptide is collisionally excited, a proton from its protonated N-terminus is free to travel along the peptide backbone and to form intermediates with protonated amide nitrogens, with protons on the side chains of arginines and lysins (e,g, in the case of tryptic ends) remaining immobile. For the most type of cross-linkers it is safe to assume that mobile protons will not jump through the cross-link and will remain localized to their original peptide chains. We should therefore, expect that the fragmentation spectrum of a typical charge 4+ cross-linked pair of tryptic peptides will resemble a sum of fragment spectra of individual charge 2+ peptides, as opposed to a fragment spectrum of a charge 4+ linear peptide. The b-y filter could be easily modified, if needed, to accommodate different types of experiments - e.g. when intensity of b-ions is enhanced or suppressed through modification of peptide N-termini. It can also be easily implemented as a c-z filter for spectra obtained using electron transfer dissociation; however, we have not yet tested its effectiveness in this setting due to the lack of standard annotated datasets of ETD spectra.
XLPM also does better job at annotating higher-charged fragments compared to other cross-linked identification algorithms. Partially, this is due to b-y filter, as the XLPM searches for a complimentary y-ion having a charge such, that the sum of charges is equal to the charge of the precursor. In fact we have not found much improvement in XLPM performance upon deconvolution of highly-charged fragments into 1+ fragments (data not shown).
False positive rate of cross-linked site identification
XLPM performs well, without sacrificing much of the analysis speed, when one of the cross-linker's functionality is non-specific and cross-linking at any amino-acid is possible. Finding cross-linked peptides in mass spectra is similar to finding a needle in a haystack. The mass spectra contain majority of non cross-linked peptides and a small number of cross-linked peptides. Target-decoy method of finding FDR has been widely acceptable in protein mass spectrometry data analysis. However, this method should be used with extra caution in cross-linking mass spectra analysis. As the expected correct matches of cross-linked peptides with the mass spectra are small, few hits from decoy may shoot up the FDR significantly. By putting stringent FDR requirements may eliminate some of the matches as false negatives.
In case of XLPM, we have used target-decoy method to calculate FDR and successfully validated the results. However, target and decoy sequences were analyzed separately, which may have boosted the score of some decoy sequences. In creating the database from protein sequences, XLPM removes database entries that do not contain any site of cross-linking, contains only one possible site of cross-linking at the N terminal of the digested fragment (forcing the miss cleavage), and digested fragments less than three amino acids. These filters decrease the difference in the characteristics of the target and decoy database. Thus, use of FDR requires caution in case of cross-linking analysis.
To validate XLPM's effectiveness in cross-linking assignment, we plotted the ROC curves with relaxed specificity of cross-linker at the second end. We plotted the ROC curves without any cut-off and 5% FDR. With the improvement in FDR, the area under curve for the ROC curve was also increased. Thus, relaxed specificity analysis can be an effective method for validation of cross-linking assignment and its usefulness at least as an auxiliary tool to the target-decoy method can not be ruled out.
XLPM is a fast and reliable algorithm for the identification of cross-linked peptide pairs. In XLPM we implemented, validated, and demonstrated utility of the unique spectral filter, which is based on the expected ratio of y-to-b ions in the fragment spectra. Furthermore, XLPM performs well in identification of the specific sites of the cross-linking, as demonstrated by the relaxed specificity ROC analysis.
The work presented in this paper is supported by NIH Grant R01 GM098922 (to Kevin Raney), and by a UAMS Cancer Pilot Grant (to Boris Zybailov), the Arkansas IDeA Network for Biomedical Research Excellence P20GM103429 (to the UAMS Proteomics core), the UA Center for Protein Structure and Function (P30GM103450), and the UAMS Center for Microbial Pathogenesis and Host Inflammatory Responses (P20GM103625), Arkansas Tobacco Settlement grant and the INBRE grants NCRR (5P20RR016460-11) and NIGMS (8 P20 GM103429-11) from NIH. We acknowledge NIH R01 grant GM098922 for the funding of the publication of this article.
XLPM is available to use on our web server at http://binf-app.host.ualr.edu/~mihir/cgi-bin/xlpm.cgi. Web server has limited options. However, if a user wants to use some other digestion enzyme, cross-linker or amino acids modifications, the user can email us with the details and we will update the web server to include the additional enzyme, cross-linker or amino acid modification. We also provide Perl code and MySQL dumps of XLPM upon request. One can customize the scripts and/or MySQL database to perform specific analysis. The code works with wide variety of enzymes, cross-linkers and amino acid modifications.
We thank Dr. K.I. Varughese for providing the coordinates for the x-ray crystal structure of Rim1.
This article has been published as part of BMC Bioinformatics Volume 15 Supplement 11, 2014: Proceedings of the 11th Annual MCBIOS Conference. The full contents of the supplement are available online at http://www.biomedcentral.com/bmcbioinformatics/supplements/15/S11.
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